Skip to main content
. 2000 Apr 11;97(8):4162–4167. doi: 10.1073/pnas.97.8.4162

Figure 2.

Figure 2

Effects of the poly(dA/dT) sequence on the preservation of the templates upon subtraction. (A) Long poly(dA) sequences cause loss of the template. DNA templates with 100 dA were subtracted with poly(dT)+ cDNA and measured by quantitative PCR. Upper band, signal from the testing templates; lower band, signal from internal PCR control template with 40 dA. The left half was the control in which no driver cDNA was added. Note that in the right half containing driver, the 100 dA templates were lost. (B) Short poly(dA) sequences preserve the template; same as above except DNA templates contained 16 dA and were subtracted with poly(dT)− cDNA. Upper band, signal from internal PCR control template with 40 dA; lower band, signal from the testing templates. p, picograms; f, femtograms. Note that in the right half containing driver, the 16 dA templates were preserved.