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Patterns of cDNA sequences generated with anchored oligo(dT) primers and reverse transcriptases. (A) Sequence ladder generated with dC-anchored oligo(dT) primer and MMLV reverse transcriptase. The poly(dA/dT)− clone contained only 11 dA (green) or 11 dT (red) residues, depending on the cloning orientation. Poly(dA/dT)+ clones contained longer dA or dT residues. (B) Summary of the results for different anchored oligo(dT) primers and reverse transcriptases.
