Figure 4.

Quantitative pattern of cDNAs generated by anchored oligo(dT)s. Poly(dT)⁻ and poly(dT)⁺ cDNAs were generated by anchored oligo(dT) or regular oligo(dT) primers and used for Southern blot analysis. The original mRNA was used for Northern blot analysis. The relative levels of a group of genes in the cDNAs and mRNA were determined by hybridization. The quantity for each gene in Northern blot analysis was set as 1, and the quantity for each gene from the Southern blot analysis was normalized to that for each corresponding gene. n, Northern blot; S, Southern blot; dT, oligo(dT) priming in reverse transcription; dN, anchored oligo(dT) priming in reverse transcription.