Figure 3.

An alignment of the N termini of G. lamblia, E. histolytica, mitochondrial, hydrogenosomal, and eubacterial cpn60 homologs. The deduced N-terminal sequence of G. lamblia and E. histolytica cpn60s are aligned with homologs from L. tarentolae, T. vaginalis, and Caulobacter crescentus. The L. tarentolae and T. vaginalis targeting peptides (underlined) are removed during import into mitochondria and hydrogenosomes, respectively (9, 39). An N-terminal extension of the E. histolytica cpn60 homolog is suggestive of a targeting peptide for a cryptic organelle in this organism. G. lamblia cpn60 has a small, 2-aa N-terminal extension relative to C. crescentus. Amino acid identities of the G. lamblia cpn60 to other homologs are indicated by asterisks (∗) under the alignment.