Figure 3.

Expression of the dystrobrevins, dystrophin, and utrophin in mouse tissue. Identical Western blots of mouse tissues were probed with the following antibodies: (Upper left) βCT-FP; (Lower left) α1CT-FP; (Upper right) MANDYS1 raised against the dystrophin rod domain; and (Lower right) MANCHO3 raised against the C terminus of utrophin. The complex pattern of proteins detected by βCT-FP correspond to muscle α-dystrobrevin 1 (94 kDa), nonmuscle α-dystrobrevin 1 (78 kDa), β-dystrobrevin (61 kDa in brain and 63 kDa in kidney), muscle α-dystrobrevin 2, and nonmuscle α-dystrobrevin 2. Dystrophin is expressed at high levels in heart and skeletal muscle whereas utrophin is ubiquitously expressed but is most abundant in the lung. Note the low level of dystrophin in brain homogenates.