Figure 3.

Northern blot analysis of the level of expression of hREV1 antisense RNA in the cell strains. RNA extracted from the two derivative cell strains, 7AGM-17C and 7AGM-12B, which had been transfected with plasmid pR1P27-AS containing the 4,117-bp sequence of the hREV1 RNA in an antisense orientation, and from their nontransfected parental cell strain, MSU-1.2-7AGM, and analyzed for expression of hREV1 antisense RNA (hREV1 AS RNA) and/or the endogenous hREV1 sense RNA. The latter mRNA, which is ≈4.4 kbp in length, can be seen as a faint band just above the antisense band in the first two lanes, and in the third lane. The lower band, approximately 1.4 kbp in length, is the endogenous HPRT mRNA, which was used to normalize the amount of RNA loaded per lane. There was no significant difference between the two derivative cell strains in the level of expression of the antisense RNA.