Figure 1.
Detection of SLC mRNA in mouse tissues by in situ hybridization. Sections were hybridized with antisense (A, B, E–G) or sense (C, D, H) 35S-labeled SLC riboprobe. (A) Dark field micrograph of SLC antisense probe hybridization in lymph node. Signal is seen as white dots. HEV are indicated by arrows. (B) SLC antisense probe hybridization in spleen. SLC sense probe does not hybridize to lymph node (C) or spleen (D). (E) High power view of HEV from A shown in bright field demonstrating typical morphology. Signal is seen as black dots. (F) Hybridization of SLC to the endothelium of small lymphatics (indicated by arrowheads) in liver. (G) Hybridization of SLC to the endothelial cells lining a central lactile (indicated by asterisks) in small intestine. (H) Lack of hybridization to SLC sense probe in small intestine. Identified structures are: F, follicle; T, T cell area; ca, central arteriole; A, artery; V, vein; bd, bile duct. [Bars = 100 μm (A–D) and 50 μm (E–H).]