Table 2.
rDEN1/4 chimeric virus replication in SCID-HuH-7 mice.
| Virus1 | No. mice | Peak virus titer (log10 PFU/ml ± SE) | Fold reduction from wild type DEN1 |
| DEN1 Puerto Rico/942 | 18 | 6.6 ± 0.1 | -- |
| rDEN1/4(CME) | 8 | 5.0 ± 0.43 | 40 |
| rDEN1/4Δ30(CME) | 7 | 4.4 ± 0.43 | 159 |
| rDEN1/4(ME) | 9 | 6.8 ± 0.3 | -2 |
| rDEN1/4Δ30(ME) | 7 | 5.2 ± 0.53 | 25 |
1 Groups of SCID-HuH-7 mice were inoculated into the peritoneal tumor with 4.0 log10 PFU of the indicated virus. Serum was collected on day 7, and virus titer was determined in Vero cells.
2 Data combined from 2 experiments.
3 Significant reduction compared to wild type DEN1 (Tukey post-hoc test, P < 0.05).