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. 2000 Apr 4;97(8):4192–4197. doi: 10.1073/pnas.050417697

Figure 4.

Figure 4

Evolution of the SIV Gag p11C-specific CTL response detected by tetramer staining and functional cytotoxicity assays in a representative vaccinated monkey. Tetramer staining of fresh PBL, tetramer staining of p11C-stimulated PBL, and functional lysis of p11C-pulsed targets by epitope peptide-stimulated PBL are shown for the following time points: week 0 (preimmune), week 2 (2 weeks after the primary immunization), week 6 (2 weeks after the second immunization), and week 10 (2 weeks after the third immunization). Staining with the Mamu-A*01/p11C tetramer was assessed by four-color flow cytometry on gated CD3+CD8+ lymphocytes. Functional lysis was determined by using p11C epitope peptide- and p11B control peptide-pulsed target cells. Tetramer staining was evident in peptide-stimulated PBL after the primary immunization and in fresh PBL after the second immunization. Functional lysis correlated well with tetramer staining. This particular monkey (772) received the DNA vaccines and IL-2/Ig protein.