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. 2006 May 25;575(Pt 1):23–35. doi: 10.1113/jphysiol.2006.106351

Figure 6. Visual characterisation of FM1-43 labelling of guinea-pig airway smooth muscle.

Figure 6

A, high-contrast representative image showing FM1-43 labelling of guinea-pig airway smooth muscle. B–G, representative photomicrographs showing FM1-43 labelling of guinea-pig trachea whole-mount preparations in the absence (control, B) or presence of 1 μm atropine (C), 100 μm acetylcholine (D), 3 μm methoctramine (E), 100 nm 4-DAMP (F) or 3 μm methoctramine plus 100 nm 4-DAMP (G). Bar chart displays quantitative analysis (mean ±s.e.m., n = 4–8 for each) of FM1-43 labelling intensity assessed from digital photomicrographic images. *P < 0.05, significantly different from control; #P < 0.05, significantly different from atropine pretreatment. Atro, atropine; ACh, acetylcholine; Methoc, methoctramine. Scale bars, 50 μm.