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. 2006 Jun 22;575(Pt 2):627–644. doi: 10.1113/jphysiol.2006.113985

Figure 8. Cardiac and vascular KATP channels are activated by 11,12-EET through different mechanisms.

Figure 8

A, recordings of whole-cell KATP currents (after subtraction of glyburide-insensitive K+ currents) from rat ventricular myocytes. Extracellularly applied 11,12-EET (5 μm) produced small enhancement of KATP currents after 10 min of exposure (left panel), while intracellularly applied 11,12-EET resulted in a much greater stimulation of current activities (right panel). Cardiac KATP currents were recorded in the presence of 140 mm intracellular K+ and 4.5 mm extracellular K+. B, vascular KATP currents recorded from rat mesentery arterial smooth muscle cells. Extracellularly applied 11,12-EET (5 μm) produced significant KATP current activation (left panel), while intracellularly applied 11,12-EET had no effect (right panel). Vascular KATP currents were recorded in the presence of symmetrical K+ concentration at 140 mm. I–V relationships of cardiac (C) (n = 6 for all groups) and vascular KATP channels (D) (n = 4 for intracellularly applied 11,12-EET and n = 8 for extracellularly applied 11,12-EET), showing the effects of 5 μm 11,12-EET applied intracellularly or extracellularly. *P < 0.05, intracellularly applied 11,12-EET versus baseline. †P < 0.05, extracellularly applied 11,12-EET versus baseline. ‡P < 0.05, intracellularly applied versus extracellularly applied 11,12-EET.