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. Author manuscript; available in PMC: 2007 Aug 7.
Published in final edited form as: Neurosci Lett. 2006 Jun 6;403(3):315–317. doi: 10.1016/j.neulet.2006.05.019

Figure 1.

Figure 1

Verification of specificity of primers used for amplification of Nav1.6 and Nav1.2 cDNAs in real-time quantitative RT-PCR. The primers were tested for specificity against plasmids containing the sequence corresponding to the C-terminus of Nav1.1 (I), Nav1.2 (II), Nav1.3 (III), and Nav1.6 (VI) (cloned in PGEM or PGEX-3X vector). Control lanes marked NT represent PCR with no added template, or with undigested template. Products were run on a 10% polyacrylamide gel.