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. 2007 Mar 21;2(3):e313. doi: 10.1371/journal.pone.0000313

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Foligne et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Result of the in vivo administration of anti-CD25 mAb (PC61) on the protective effect of probiotic-pulsed BMDCs in TNBS-induced colitis. Ten mice per group were challenged with TNBS alone (CTL) or after Lr32-pulsed BMDC transfer (Lr32-DC). The effect of a pre-treatment by either 200 µg of anti-CD25 (α-CD25) or control rat IgG (rat IgG) mAb, 24 h before colitis induction was analyzed in mice treated or not by Lr32-pulsed BMDC (Lr32-DC) transfer. Wallace inflammation scores (mean ± SEM) were calculated in each group and compared to each other by a Mann-Whitney U test (<0.01, **; p<0.001, ***). Percent of mortality and protection (reduction of the mean Wallace scores of mice treated with BMDCs, in relation to the mean score of TNBS control group) are also reported.