Table I. Apparent sedimentation coefficient of ClpB and its truncated forms at low and high protein concentration.
Sedimentation velocity data shown in Fig. 3 were analyzed with the time-derivative method (19). The observed apparent sedimentation coefficients were obtained from the maxima of the apparent sedimentation coefficient distributions (Fig. 4). The observed sedimentation coefficients were corrected to values corresponding to the density and viscosity of water (see Experimental Procedures) and are reported as s20,w in Svedberg units (S). The data for wild type ClpB (0.1 mg/ml and 4.0 mg/ml) are from Ref. 9.
| Protein Sample | s20,w (S) | |
|---|---|---|
| 0.2 mg/ml protein | 4.0 mg/ml protein | |
| ClpB | 4.7 | 16.4 |
| ClpBΔN | 4.5 (minor component)
13.8 (major component) |
14.8 |
| ClpBΔC | 4.2 | 3.9 |
| wt ClpB | 4.4 | 17.1 |