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Gel filtration chromatography of the N-terminal fragment of ClpB. An aliquot of the N-terminal fragment (20 μL, 1.0 mg/mL) was injected onto a Superose 6 column. Protein elution profile was obtained with a 0.05 mL/min flow-rate in 50 mM Tris-HCl (pH 7.5), 0.2 M KCl, 20 mM MgCl2, 1 mM EDTA, and 1 mM DTT. (•) Elution times of thyroglobulin (Mr 670,000`), γ-globulin (Mr 158,000), ovalbumin (Mr 44,000), myoglobin (Mr 17,000), and vitamin B-12 (Mr 1350).
