Fig. 4.

Differential scanning calorimetry (DSC) of the N-terminal fragment of ClpB. (A) Shown are the DSC data obtained with a 1 K/min scan-rate for the dialysate buffer (broken line) and for the N-terminal domain of ClpB (2.0 mg/mL in 50 mM Tris-HCl at pH 7.5, 0.2 M KCl, 20 mM MgCl₂, 10% glycerol, 1 mM EDTA, 1 mM DTT) during the first scan (thick solid line), second scan (thin solid line), and third scan (dotted line). (B) DSC data from the first scan of the N-terminal domain of ClpB (solid line) after subtraction of the buffer scan and a linear baseline in the region of the thermal transition with a two-state model fit of the data (broken line).