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ATPase activity of ClpB, ClpBΔN, and ClpBΔN with the N-terminal fragment of ClpB. The rate of ATP hydrolysis has been measured by incubating the ClpB variants (5.6 pmole ClpB, 6.3 pmole ClpBΔN without and with 1.35 nmole N-terminal fragment) for 15 min at 37°C in 50 μL of the assay buffer (see Materials and Methods) in the absence of other proteins (black bars), with 0.2 mg/mL κ-casein (grey bars), or with 0.01 mg/mL poly-L-lysine (hatched bars).
