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The kinetics of the appearance of spontaneously secreting, IgG-AFC in peripheral blood of two adult volunteers after a booster immunization with a pneumococcal conjugate vaccine (Pnc7), 1 year after a primary dose. The IgG-AFC specific for tetanus toxoid (a) diphtheria toxoid (b) serotype-4 (c) serotype-14 (d) and serotype 23F (e) enumerated by ELISpot are shown. The data are expressed as the number of antigen specific IgG-AFC per million PBMC.
