Figure 3.
Pam3Cys does not induce Ca2+ mobilization or degranulation in MLMC and BMMC. (a) MLMC or (b) BMMC were loaded with Indo-1AM, stimulated with Pam3Cys (10 µg/ml) or DNP-BSA (20 ng/ml) and intracellular calcium was measured. (c) MLMC and (d) BMMC were stimulated with Pam3Cys (10 µg/ml) or DNP-BSA (20 ng/ml) for 30 min and β-hexosaminidase release was determined. Data shown are representative of three similar experiments.