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. 2006 Nov;119(3):421–429. doi: 10.1111/j.1365-2567.2006.02453.x

Figure 3.

Figure 3

Stimulation of GITR induces cytokine/chemokine expression in monocyte/macrophage cell lines. (a) THP-1 cells were stimulated with immobilized anti-hGITR mAb or mIgG. Culture supernatants were collected 24 hr after activation and cytokine concentrations were measured using ELISA. Concentrations used for the immobilization were 200, 20 and 2 μg/ml for the anti-GITR mAb and 200 μg/ml for mIgG. (b,c) RAW264.7 cells were stimulated with anti-mGITR mAb added to the culture medium at 30, 10 and 1 μg/ml concentrations or 30 μg/ml of rat IgG (b) or rmGITRL added at 10, 3 and 1 μg/ml concentrations (c). Culture supernatants were collected 48 hr after activation and TNF-α concentrations were measured using ELISA. C, treatment with isotype-matching control antibody; N, no treatment control; L, treatment with 1 μg/ml lipopolysaccharide as a positive control. Mean values of triplicate measurements ± SD are shown.