Figure 1.
HVEM activates the respiratory burst, release of IL-8 and degranulation, but not the phagocytosis of PMN. PMN (2 × 105/well) were cultured on titrated amounts of anti-HVEM-coated 96-well culture plates. (a) PMN were cultured for 145 min in the presence of DCFH-DA for the measurement of respiratory burst. The mean + SD of one representative experiment performed in triplicate wells is shown. (b) Supernatants of PMN activated with the indicated stimuli were collected after 18 hr and analysed for the release of IL-8 by ELISA. Mean + SD of one representative experiment performed in triplicate wells is shown. (c) Surface expression of CD66b was analysed by flow cytometry after a 60-min incubation on anti-HVEM (10 μg/ml) coated (filled histogram) or control mouse IgG1 (10 μg/ml) coated plates (open histogram). (d) Phagocytosis of phycoerythrene-labelled polystyrene beads after 1 hr incubation on anti-HVEM (10 μg/ml) coated (filled) or control mouse IgG1 (10 μg/ml) coated plates (open). All results shown are representative of at least three independent experiments with different donors.