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HVEM does not affect TLR- or GM-CSF-mediated survival of PMN. PMN (2 × 105/well) were cultured on anti-HVEM or control mAb-coated (10 μg/ml) plates in the presence of LPS 500 ng/ml, Pam3Cys 500 ng/ml, imiquimod 10 μg/ml or GM-CSF 100 U/ml. The amount of apoptotic cells was analysed by DNA fragmentation as described in the Materials and methods after 24 hr. A compiled analysis of three dependent experiments each performed in duplicates is depicted.
