Table 3.
Results of MALDI-TOF-MS and Database Search for Differentially Expressed Proteins in VArasmut Cells.
| Protein Name | Taxonomy* | Accession Number | Mascot Score† | Peptides Matched | Peptide Coverage (%) | Molecular Weight Mw (Theoretical/Experimental; kDa) | pI (Theoretical/Experimental) | VArasmut/VA‡ | ± SD |
| Keratin type II, cytoskeletal 8 | B | P05786 | 239 | 17 | 55 | 42.2/53.3 | 5.13/5.92 | +3.3 | ±0.3 |
| Stress-induced phosphoprotein 1 | M | AAH03794 | 102 | 10 | 20 | 63.2/64.6 | 6.40/6.27 | +3.0 | ±1.2 |
| Heat shock 70-kDa protein 8 | B | NP_776770 | 133 | 12 | 24 | 71.4/71.5 | 5.49/5.50 | +2.8 | ±0.9 |
| Heat shock 27-kDa protein | C | P42929 | 92 | 7 | 30 | 22.9/25.0 | 6.23/6.11 | +2.6 | ±1.0 |
| Heat shock 70-kDa protein 8 isoform | B | NP_776770 | 66 | 7 | 12 | 71.4/71.5 | 5.49/5.45 | +2.3 | ±0.2 |
| Glucose-regulated protein 58 kDa | B | NP_776758 | 280 | 20 | 42 | 57.3/58.6 | 6.23/6.16 | +2.1 | ±0.4 |
| Annexin 1 | B | NP_786978 | 190 | 13 | 48 | 39.1/35.8 | 6.44/6.39 | +1.9 | ±1.4 |
| Rho GDP dissociation inhibitor α | B | NP_788823 | 129 | 8 | 37 | 23.5/26.5 | 5.12/5.24 | +1.8 | ±0.5 |
| β/γ Actin | M | CAA31455 | 95 | 8 | 26 | 41.3/44.1 | 5.56/5.43 | +1.8 | ±1.0 |
| Heat shock 70-kDa protein 5 | H | NP_005338 | 147 | 12 | 24 | 72.4/75.2 | 5.07/5.12 | +1.6 | ±0.2 |
| β Tubulin [fragmented protein] | M | NP_076205 | 197 | 14 | 27 | 50.4/36.0 | 4.78/5.59 | +1.6 | ±0.6 |
| γ Actin | H | AAA51580 | 81 | 5 | 21 | 26.1/26.1 | 5.65/5.65 | -1.6 | ±0.7 |
| α Tubulin [fragmented protein] | H | NP_116093 | 93 | 8 | 26 | 50.5/38.3 | 4.96/5.67 | -1.8 | ±0.7 |
| Tropomyosin 1 | H | NP_000357 | 105 | 9 | 25 | 32.8/31.2 | 4.81/4.69 | -1.9 | ±0.8 |
| Keratin, type II cytoskeletal 8 [fragmented protein] | B | P05786 | 115 | 8 | 25 | 42.4/26.4 | 5.13/5.01 | -2.0 | ±1.1 |
| Vimentin | B | NP_776394 | 187 | 15 | 42 | 53.7/47.7 | 5.20/4.89 | -2.1 | ±0.7 |
| β/γ Actin | B | ATBOB | 86 | 6 | 20 | 41.9/41.9 | 5.31/5.29 | -2.1 | ±1.2 |
| Vimentin | B | NP_776394 | 130 | 11 | 31 | 53.7/44.9 | 5.20/4.68 | -2.5 | ±0.9 |
| Myosin regulatory light chain | H | P_006462 | 60 | 4 | 29 | 19.8/21.2 | 4.67/4.60 | -3.0 | ±0.2 |
| Proliferating cell nuclear antigen | M | NP_035175 | 89 | 6 | 26 | 29.1/32.7 | 4.66/4.60 | -5.0 | ±0.9 |
pI = isoelectrical point; SD = standard deviation.
*
C = Canis familaris; B = Bos taurus; H = Homo sapiens; M = Mus musculus.
†
The mascot score is measured as -10*log(P), where P is the absolute probability that the observed match is a random event.
‡
Fold change: (+) an increase in protein expression in VArasmut cells; (-) a decrease in protein expression in VArasmut cells.