Fig. 11.

Unrooted radial phylogenetic tree of C. elegans gst coding sequences constructed by the maximum likelihood method, as implemented in the software fastDNAml (Olsen et al., 1994), and visualized using TreeView (Page, 1996). The numbers refer to gst gene designations. The 5 GSTs which we identified as having 4-HNE-conjugating activity are shown on black background; of these, the 2 enzymes (gst-5 and gst-10) whose knock-down by RNAi leads to life span shortening are boxed. The length of 3 branches in the tree (marked with filled circles) may not be significantly greater than zero (P ≥ 0.05), and the length of one branch (marked with open circle) is positive at 0.01 ≤ P < 0.05; all other branches are significant at P < 0.01. The scale bar represents a branch length corresponding to an evolutionary distance equivalent to one expected nucleotide substitution at any given site.