Table 1.

Peptides from ESAT-6

HLA class I allele	Peptide motif	Peptide	Sequence	Position
HLA-A2	-L/I/M------V/L/I	ES8	GIEAAASAI	10–18
		ES9	AIQGNVTSI	17–25
		ES10	LLDEGKQSL	28–36
		ES11	ELNNALQNL	64–72
		ES13	AMASTEGNV	82–90
HLA-B8	--K-K---L/I	ES7	EGKQSLTKL	31–39
HLA-B52	-Q-----I/V	ES12	LQNLARTI	69–76

We searched for 8-, 9-, or 10-residue sequences from ESAT-6, which were congruent with the peptide motifs of the HLA class I alleles -A2, -B7, -B8, -B35, -B52, and -B53. Sequences congruent with the peptide motifs for HLA-A2, HLA-B8, and HLA-B52 were identified; these peptides were synthesized and are displayed. No sequences congruent with HLA-B7, HLA-B35, and HLA-B53 were present in ESAT-6 and, thus, no peptides were synthesized for these HLA class I alleles. Peptides were sorted into pools that were used for in vitro restimulation of donor PBMCs. Peptides found to be CD8⁺ epitopes are shown in boldface. Similarly, 42 peptides were synthesized based on the sequences of antigens 85A and 85C. No CD8⁺ epitopes were identified among these and the peptides are not shown.