Table 1.
Expression of FasL cytotoxicity and transfer of G418 resistance by fractions of hFasL-PA317 supernatant
| Exp. | Total units in fraction (% of CFS activity)
|
|||
|---|---|---|---|---|
| CFS | VFS | VP | ||
| FasL cytotoxicity, units* | 1 | 1,225 (100%) | 525 (43%) | 525 (43%) |
| 2 | 1,330 (100%) | 490 (37%) | 600 (45%) | |
| 3 | 1,440 (100%) | 560 (39%) | 700 (49%) | |
| Transfection activity, cfu† | 1 | 3.9 × 106 (100%) | 0 (0%) | 1.0 × 106 (26%) |
| 2 | 9.5 × 106 (100%) | 0 (0%) | 3.5 × 106 (37%) | |
Culture supernatant (35 ml) from the hFasL-PA317 virus-packaging cell line was used to prepare CFS, VP, and VFS as described in Materials and Methods.
*
One unit of FasL cytotoxicity is defined as the amount required to cause 20% specific 51Cr release in a 5-h 51Cr-release assay against LB27.4 targets.
† cfu, colony-forming unit; defined as the unit required to transfer G418 resistance in NIH 3T3 cells and to produce one viable colony.