FIG. 4.

ChIP analyses of the transposon locus. Formaldehyde cross-linked chromatin from tTR (no. 1) and control (no. 1) ES clones cultured with DOX for 3 days to exclude tTR from tetO was immunoprecipitated without antibody (No Ab) or with anti-dimethyl H3-K9, anti-trimethyl H3-K9, anti-dimethyl H3-K4, or anti-acetyl H3 antibody. Fivefold serial dilutions of input and precipitated DNAs from tTR and control clones were used for semiquantitative PCR. Oct4 and Mage-a2 were used as controls for euchromatic and heterochromatic regions, respectively.