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. 2007 Jan 8;27(5):1844–1858. doi: 10.1128/MCB.01363-06

FIG. 5.

FIG. 5.

XCPE1 variants containing multiple base changes from the wild-type HBV X promoter sequence retain specific promoter activities. The XCPE1 sequences that are 2-, 3-, and 4-nucleotide divergent from the HBV sequence were made by site-directed mutagenesis of pBS-HBXB (HBV enhancer and X promoter-containing construct) and analyzed for the promoter activity. In vitro transcription was carried out with HepG2 nuclear extract. Lanes 2, 3, 4, and 6 show the results of the transcription from the templates containing base changes from the wild-type HBV X promoter sequence at nt 1113 and 1116; nt 1111, 1113, and 1116; nt 1111, 1113, 1116, and 1118; and nt 1113, 1116, and 1119, respectively. Dots above the XCPE1 sequences indicate positions of the observed start sites.