FIG. 2.

Subcellular localization of BBF2H7. HeLa cells transfected with BBF2H7 were incubated in the presence or absence of 1 μM thapsigargin (TG) or 10 μM MG132 for 6 h and then costained with anti-BBF2H7 and anticalnexin antibodies. Note that BBF2H7 immunoreactivity completely overlaps that of calnexin under normal conditions, but after ER stress or proteasome inhibition, the BBF2H7 signal accumulates in the nucleus. The lower panel shows that the BBF2H7 deletion-mutated protein lacking the transmembrane and luminal domains (BBF2H7-376) localized in the nucleus under normal conditions.