TABLE 1.
Absolute numbers of lymphocytes in thymus, spleen, and lymph nodesa
| Organ | ptαCre+ CKO (n = 10) | ptαCre+ control (n = 9) | Cre− control (n = 15) |
|---|---|---|---|
| Thymus | |||
| DN2 + 3 | 0.71 ± 0.41 | 1.4 ± 1.6 | 1.6 ± 1.5 |
| DN4 | 0.07 ± 0.05 | 0.68 ± 1.1 | 0.95 ± 0.78*** |
| DP | 3.3 ± 2.7 | 69.0 ± 47.1** | 109 ± 52.6*** |
| CD4SP | 0.86 ± 0.59 | 13.4 ± 4.9*** | 38.3 ± 39.3** |
| CD8SP | 0.30 ± 0.17 | 2.9 ± 1.4*** | 2.8 ± 1.2*** |
| TCRγδ | 0.11 ± 0.08 | 0.44 ± 0.31* | 0.61 ± 0.50** |
| Spleen | |||
| CD4+ | 4.7 ± 2.9 | 22.8 ± 9.3*** | 23.7 ± 9.0*** |
| CD8+ | 2.0 ± 1.3 | 10.2 ± 4.2*** | 8.7 ± 5.5*** |
| CD19+ | 26.6 ± 8.5 | 40.1 ± 19.9 | 29.9 ± 11.4 |
| TCRγδ | 0.47 ± 0.32 | 0.66 ± 0.37 | 0.73 ± 0.25 |
| Lymph nodes | |||
| CD4+ | 4.8 ± 3.0 | 21.8 ± 11.4** | 27.7 ± 9.2*** |
| CD8+ | 2.1 ± 1.1 | 8.8 ± 4.3** | 8.2 ± 5.6*** |
| CD19+ | 10.1 ± 4.3 | 7.7 ± 5.2 | 8.3 ± 4.0 |
| TCRγδ | 0.33 ± 0.13 | 0.41 ± 0.40 | 0.37 ± 0.20 |
a
Absolute numbers (106 ± SD) of the indicated cell populations are shown. DN cells were CD4− CD8−, DP were CD4+ CD8+, CD4SP were CD4+ CD8−, and CD8SP were CD4− CD8+. Asterisks indicate the P values (CKO versus Cre+ control and Cre− control mice, respectively). *, P = 0.05 to 0.01; **, P = 0.01 to 0.001; ***, P < 0.001. n, number of mice.