TABLE 2.
Cell subpopulations assayed for actively dividing and apoptotic cellsa
| Strain | % of cells in S phase ± SD
|
% of annexin V+ cells ± SD
|
% TUNEL+ cells ± SD
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of mice | L cells | DN4 | DP | CD4SP | CD8SP | No. of mice | L cells | DN4 | DP | CD4SP | CD8SP | No. of mice | DN | DP | CD4SP | |
| PtαCre+ CKO | 8-12 | 30.8 ± 9.7 | 22.4 ± 7.7 | 6.3 ± 1.7 | 1.8 ± 1.3 | 4.6 ± 4.7 | 9 | 5.6 ± 3.1 | 55.9 ± 6.4 | 6.7 ± 4.9 | 18.6 ± 9.2 | 5.4 ± 2.7 | 8 | 3.3 ± 1.1 | 0.83 ± 0.21 | 0.18 ± 0.08 |
| ptαCre+ control | 9-13 | 42.0 ± 8.7* | 41.0 ± 10.5*** | 4.5 ± 1.4** | 1.2 ± 0.9 | 4.5 ± 4.5 | 10 | 2.3 ± 1.3* | 54.7 ± 8.0 | 2.1 ± 0.8* | 4.3 ± 2.4** | 3.6 ± 1.6 | 9 | 3.0 ± 1.2 | 0.42 ± 0.12*** | 0.10 ± 0.03* |
| Cre− control | 7 | 50.1 ± 9.2** | 48.6 ± 10.9*** | 4.8 ± 0.8* | 1.0 ± 0.3 | 3.1 ± 1.8 | 16 | 1.5 ± 0.8** | 43.3 ± 10.6** | 1.6 ± 0.3* | 3.2 ± 1.1*** | 2.8 ± 1.7* | 9 | 3.0 ± 1.3 | 0.30 ± 0.09*** | 0.11 ± 0.04* |
a
Percentages of cells in the S phase and cells positive for annexin V and TUNEL. For details see the legend for Fig. 3. Asterisks indicate the P values (CKO versus Cre+ control and Cre− control mice, respectively). *, P = 0.05 to 0.01; **, P = 0.01 to 0.001; ***, P < 0.001.