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. 2006 Dec 18;27(5):1602–1613. doi: 10.1128/MCB.01956-06

FIG. 2.

FIG. 2.

Nucleosomes are repositioned to sites more distal from the DSB. Chromatin DNA isolated from cells induced with 1.0 h of HO expression (+) or with no induction (−) was partially digested with MNase and then deproteinized and fully digested with BamHI (a), HindIII (b), or EcoRI (c) to yield common end fragments. Southern blot hybridization was carried out using a radiolabeled probe that anneals 2 kb distal to the break (a) (+1680 to +2313 with respect to the HO cut), 1 kb distal (b) (+337 to +1211 relative to the HO cut), or 2 kb proximal (c) (−2606 to −2034 relative to the HO cut) to map the nucleosome positions adjacent to a DSB at the MAT locus. Molecular weight markers (M) and the MNase digest of naked DNA (naked) are shown for comparison. The arrows indicate the locations of the HO breaks. The inferred nucleosome positions are shown next to the panels of indirect end labeling.