FIG. 5.

Lack of RSC reduces the chromatin remodeling at the DSB. The MNase cleavage profiles of the tet-Sth1 (SLY579) with 10 μg/ml doxycycline (A) or SLY403 (rsc2Δ) (B) are shown at 0, 0.5, and 1.0 h after galactose was added to the culture medium as described in Fig. 1. Inferred nucleosome positions before and after the DSB are shown below each graph. Each oval represents an individual nucleosome; the dotted ovals are the nucleosomes lost upon DSB induction. The arrow indicates the location of the HO-induced break. The average for three independent experiments is shown.