TABLE 1.

Summary of mutations and their effect on telomere function in vivo

Element	Name	Position	Sequence (5′ to 3′)		Colony phenotypea	Telomere length (% of WT)
			WT	Mutation
Stem 1	S1.1	880-881	AA	GG	1	100
	S1.1′	858-859	UU	CC	2	50
	S1.1+1′				1	100
	S1.2	880-883	AACC	UUGG	3	30
	S1.2′	856-859	GGUU	CCAA	1-2	63
	S1.2+2′				1	94
Bulge	BtS1	957-959	CAU	GGA	1	90
	BtS1′	876-878	UCC	AUG	1	90
	BtS1+1′				4	Recb
	ΔB	876-878	UCC	DEL	4	Rec
	BtS2	958	A	G	1	90
	BtS2′	876-878	UCC	GCG	1	90
	BtS2+2′				1	85
Stem 2	S2.0	960-962	AAA	GGG	4	30
	S2.0′	873-875	UUU	CCC	4	40
	S2.1	964-966	UCA	AGU		30-40
	S2.1′	869-871	UGA	ACU		30-40
	S2.2	961-963	AAA	UUU		Rec
	S2.2′	872-874	UUU	AAA		Rec
	S2.3	961-963	AAA	GGG		Rec
	S2.3′	872-874	UUU	CCC		Rec
	S2.4	961-963	AAA	CCC		Rec
	S2.4′	872-874	UUU	GGG		Rec
	S2.5	961-966	AAAUCA	GGGAGU		Rec
	S2.5′	869-874	UGAUUU	ACUCCC		Rec
	S2.5+5′					60
	S2.6	964-966	UCA	CCG		50
	S2.7	964-967	UCAU	CCGC		70
	S2.6′	869-871	UGA	CGG		50
	S2.6+6′				1	100
	S2.7+6′				1	90
Loop	L.a	862-864	UUU	CCC
	L.b	863-865	UUU	CCC	3	Rec
	L.c	864-866	UUU	CCC	3-4	Rec
	L.d	864-866	UUU	AAA	3-4	Rec
	L.e	866	U	C	1	100
Other	S2.0′+L.b				4	Rec
	S2.0′+L.a				4	Rec
	S2.3′+L.c				4	Rec
	S2.3+L.c				4	Rec

^aColony phenotypes are rated according to the severity of the phenotype from 1 (the smooth appearance of wild-type strains) to 4 (the severe, rough phenotype observed in a terΔ strain).

^bRec, deregulated telomere length typical of the alternative, telomerase-independent recombination pathway. Such a phenotype is observed in a terΔ strain after its telomeres shorten to a critical length.