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. 2007 Jan 8;27(6):2398–2410. doi: 10.1128/MCB.01509-06

FIG. 5.

FIG. 5.

(A) Histone H1 enhances basal MMTV transcription only at very high H1/N ratios. S1-nuclease analysis of MMTV-driven transcription of groups of 8 oocytes injected with 2.8 ng of GR mRNA and increasing amounts of H1 mRNA to achieve the apparent H1/N ratios indicated was done according to the procedures illustrated in Fig. 1C. No hormone was added (TA). (B) Hormone-activated MMTV transcription is enhanced at an apparent H1/N ratio of ∼1 and is partially inhibited at higher levels. S1-nuclease analysis of MMTV transcription of duplicate pools of eight oocytes injected with mRNA mixes containing 3.7, 0.8, and 3.2 ng of GR, NF1, and Oct1 mRNA, respectively, and amounts of H1 mRNA to achieve the apparent H1/N ratios indicated was done according to the procedures illustrated in Fig. 1C. (C) An apparent H1/N ratio of ∼1 enhances hormone-dependent MMTV transcription. The experiment was as described for Fig. 4B but used triplicate pools of eight oocytes and injection with mRNA mixes containing 1.2, 0.9, and 2.3 ng of GR, NF1, and Oct1 mRNA, respectively.