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. 2007 Jan 22;27(6):2048–2058. doi: 10.1128/MCB.01100-06

FIG. 2.

FIG. 2.

Silencing c-Myb expression decreases cyclin B1 expression. (A) c-myb-1 and c-myb-2 siRNAs against c-myb were transfected into Mo7e cells as described in Materials and Methods. Protein expression was determined by Western blotting 24 h after transfection. (B) B-myb and control siRNA duplexes (5 μg) were transfected into Mo7e cells as described in Materials and Methods. Protein expression was then determined by Western blotting 24 h posttransfection. (C) Western blot analyses of cyclin B1, cyclin A, cdc2, cyclin D1/2, and cyclin E protein expression in K562-MERT cells following 1 μM 4-OHT treatment for 0, 24, and 48 h.