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. 2007 Jan 22;27(6):2309–2323. doi: 10.1128/MCB.01875-06

FIG. 2.

FIG. 2.

Subcellular locations of EKLF. 293T cells (top row), blood islands of E9.5 mouse yolk sacs (second row), peripheral blood cells of E10.5 AGM (third row), and E14.5 fetal liver cells (bottom row) were stained with AEK-1 and examined with the confocal microscope. The broken lines in the first column indicate the boundaries of the nuclei, as defined by the DAPI staining. Lower-magnification pictures are shown in the last column. The percentage values represent the fractions of EKLF-positive cells containing the majority of EKLF (more than 90%) in the nucleus. n values represent the numbers of cells analyzed for each set of the experiments. Each set consists of three or more independent analyses. Western blot analysis results of the nuclear (Nu) and cytoplasmic (Cyto) extracts prepared from the blood island cells of E9.5 yolk sacs, peripheral blood cells of the E10.5 AGM, and E14.5 fetal liver cells are shown in the lower panel, with tubulin as the cytoplasmic marker and hnRNP A1 as the nuclear marker.