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NRG1 stimulates Nrdp1 accumulation. (A) MCF7 cells were treated without or with NRG1 for 6 h, and lysates were immunoblotted with antibodies to ErbB3, Nrdp1, and actin. (B) 293T cells were cotransfected with both ErbB2 and ErbB3, GFP, or FLAG-Nrdp1, as indicated. Cells were treated without and with NRG1 for 6 h, and lysates were blotted with antibodies to phosphotyrosine (pY), phospho-Akt (pAkt), GFP, FLAG, and actin.
