FIG. 3.

USP8 knockdown suppresses NRG1-stimulated Nrdp1 accumulation. (A) 293T cells were cotransfected with V5-USP8 or its vector and USP8 shRNA or its vector, and lysates were blotted with antibodies to V5 epitope and actin. (B) 293T cells were cotransfected with ErbB2/ErbB3 and FLAG-Nrdp1 without and with USP8 shRNA plasmid, and lysates were blotted with antibodies to FLAG tag or actin. Blots were quantified, and the graph depicts the average (±standard error [SE]) stimulation of NRG1-induced Nrdp1 accumulation over three independent experiments. (C) MCF7 cells stably transduced with an empty vector or the USP8 shRNA vector were treated with NRG1, and lysates were blotted with the indicated antibodies.