FIG. 6.

USP8 association with Akt. (A) Lysates from 293T cells cotransfected with HA-Akt and V5-USP8 were immunoprecipitated (IP) with anti-V5. Lysates and precipitates were blotted with the indicated antibodies. (B) Serum-starved MCF7 cells were treated without and with NRG1 for 15 min, and endogenous USP8 was visualized by immunofluorescence. (C) V5-USP8 (green) and HA-Akt (red) in cotransfected COS7 cells were visualized by deconvolving immunofluorescence microscopy. White arrows point to regions of colocalization at the cell surface and in a perinuclear structure.