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. 2007 Jan 8;27(6):2180–2188. doi: 10.1128/MCB.01245-06

FIG. 7.

FIG. 7.

Regulation of Nrdp1 and ErbB3 ubiquitination by USP8. (A) 293T cells were cotransfected with Nrdp1 and HA-ubiquitin (HA-Ub) along with wild-type USP8, the C748A mutant, or their vector pcDNA3.1 or with shRNA USP8 or its vector. Cells were treated with MG132 to stabilize Nrdp1, and Nrdp1 immunoprecipitates (IP) were blotted with antibodies to the HA epitope and ubiquitin. (B) MCF7 cells were treated for various times with NRG1, and ErbB3 immunoprecipitates were blotted with antibodies to ubiquitin and ErbB3. (C) MCF7 cells stably transduced with the pSuper vector, Nrdp1 shRNA, or USP8 shRNA were treated for the indicated times with NRG1, and ErbB3 immunoprecipitates were blotted for ubiquitin and ErbB3.