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. 2007 Jan 22;27(6):2144–2154. doi: 10.1128/MCB.02347-06

FIG. 5.

FIG. 5.

Single-ion GC scans showing the modifying prenyl and acyl groups of lipid cleaved from His6-GFP-AtROP6 purified from the TSM and the DRM. (A, C, and E) Ion 71 gel chromatograms of farnesol (2,6,10-trimethyldodecane) and geranylgeraniol (2,6,10,14-tetramethylhexadecane) initial standards (A), lipids cleaved from NP-40-soluble purified His6-GFP-AtROP6 (AtROP6-TSM) (C), and NP-40-insoluble, SDS-soluble purified His6-GFP-AtROP6 (E) by sequential hydrogenation and Raney nickel cleavage. (B, D, and F) Ion 101 gel chromatograms showing palmitic acid (ethyl palmitate) and stearic acid (ethyl stearate) initial standards (B). Acyl lipid cleaved from NP-40-soluble purified His6-GFP-AtROP6 (AtROP6-TSM) (D) and NP-40-insoluble, SDS-soluble purified His6-GFP-AtROP6 (F). AtROP6 purified from DRM was modified by palmitic and stearic acids (F), but no cleaved acyl lipids were detected in AtROP6 purified from the TSM (D).