FIG. 6.
Activated His6-GFP-Atrop6CA was modified by both prenyl and acyl lipids. Total-ion chromatograms of the initial standard (A) and lipids extracted from purified His6-GFP-Atrop6CA (Atrop6CA) by sequential hydrogenation and Raney nickel cleavage (B) are shown. AtROP6 was modified by both prenyl and acyl lipids. Peaks: 1, 2,6,10-trimethyldodecane, saturated derivative of farnesol; 2, 2,6,10,14-tetramethylhexadecane, saturated derivative of geranylgeraniol; 3, ethyl palmitate, derivative of palmitate formed following hydrogenation; 4, ethyl stearate, derivative of stearate formed following hydrogenation. (C to F) Single-ion GC scans corresponding to the total-ion chromatogram shown in panels A and B, showing the modifying prenyl and acyl groups. (C and E) Ion 71 gel chromatograms of farnesol and geranylgeraniol initial standards (C) and farnesol and geranylgeraniol cleaved from purified His6-GFP-Atrop6CA (Atrop6CA [ion 71]) (E). (D and F) Ion 101 gel chromatograms of palmitic and stearic acid initial standards (D) and lipids cleaved from purified His6-GFP-Atrop6CA (Atrop6CA [ion 101]) (F).