FIG. 6.

HP1βΔN blocks the relocalization of HP1 proteins into PML bodies but has no effect on recruitment of HIRA into PML bodies. (A) WI38 cells were infected with vector control (Cont or Con) or HP1βΔN-expressing virus, together with a virus containing an activated Ras oncogene. The infected cells were selected with 3 μg/ml puromycin and 500 μg/ml G418 cells. Eight days postinfection, the cells were stained with antibodies to HP1β and PML. Yellow arrowheads indicate colocalizing HP1β and PML. Yellow arrows indicate HP1β in SAHF. (B) One hundred cells shown in panel A were scored for colocalizing HP1β and PML. (C) As in panel A but stained with antibodies to HIRA and PML. (D) One hundred cells shown in panel C were scored for colocalizing HIRA and PML.