Skip to main content
. Author manuscript; available in PMC: 2008 Feb 21.
Published in final edited form as: J Natl Cancer Inst. 2007 Feb 21;99(4):309–321. doi: 10.1093/jnci/djk053

Fig. 2.

Fig. 2

Intracellular localization of KISS1 to the Golgi complex of transfected C8161.9 melanoma cells. Each column represents a different C8161.9 transfectant. KGM = C8161.9 cells transfected with KISS1 into which enhanced green fluorescent protein (GFP) was inserted N-terminally to metastin. Vector = C8161.9 cells transfected with empty vector. KFM = C8161.9 cells transfected with KISS1 into which the FLAG epitope was inserted N-terminally to metastin. KFMΔSS = C8161.9 cells transfected with KFM lacking the signal sequence. Cells were stained by immunofluorescence with antibodies against FLAG and/or GM130 or giantin, as indicated. For the KGM transfectants, staining for FLAG was omitted; the fluorescence represents GFP. KFM and KGM colocalize with GM130 and giantin, but KFMΔSS shows diffuse cytoplasmic staining. Scale bar = 16 μm.