Fig. 6.

KISS1 suppression of metastasis to multiple organs in mice. Mice were injected in the left cardiac ventricle with C8161.9 cells that had been transfected with empty vector or with FLAG-tagged KISS1 containing (KFM) or lacking (KFMΔSS) the signal sequence and then infected with a green fluorescent protein–containing lentivirus. A) Representative lung, eye, kidney, and bone photomicrographs and fluorescent photomicro-graphs showing metastatic lesions 5 weeks after injection. Most extra-pulmonary sites were devoid of green cells. Scale bar = 2000 μm. B) Graphical mapping of the distribution, relative size, and frequency of fluorescent metastatic foci in bone. Bone maps reflect a data merge of all mice (10 mice per experimental group) from two or three independent experiments. Mice received C8161.9 and C8161.9^vector cells (controls); C8161.9^KFM clones 46, 57, and 59 (KFM); and C8161.9^KFMΔSS clones 6, 7, and 16 (KFMΔSS). The total number of mice examined is shown. A frequency scale is depicted above bone schematic diagrams. If more than 10 mice in a group had metastases at a particular location, the scale reads red .