Table 1.
C8161.9 metastatic incidence and frequency in multiple organs*
| Metastases |
||||||||
|---|---|---|---|---|---|---|---|---|
| Mean (95% confidence interval) |
||||||||
| Lung |
Bone |
Kidney |
Eye |
|||||
| Cells | Incidence†(%) | Size (μm) | Incidence (%) | Frequency‡ | Incidence†(%) | Frequency‡ | Incidence†(%) | Frequency‡ |
| Parental C8161.9 cells | 100 | 1020 (922 to 1117) | 100 | 7.9 (5 to 11) | 92 | 9.7 (5 to 15) | 77 | 1.3 (1 to 2) |
| Empty vector transfectant | 100 | 930 (833 to 1027) | 100 | 11.9 (11 to 18) | 93 | 14.7 (7 to 23) | 53 | 0.7 (0 to 1) |
| KFM46 | 15§ | 34§ (27 to 41) | 44§ | 0.6§ (0 to 1) | 0§ | 0∥ (−) | 0¶ | 0∥ (−) |
| KFM57 | 2§ | 25§ (22 to 28) | 10§ | 0.1§ (−) | 0§ | 0§ (−) | 0¶ | 0∥ (−) |
| KFM59 | 6§ | 27§ (24 to 31) | 20§ | 0.1§ (0 to 1) | 0§ | 0§ (−) | 0¶ | 0∥ (−) |
| KFMΔSS6 | 100 | 1148 (1053 to 1244) | 100 | 12.4 (8 to 17) | 100 | 22.6 (6 to 39) | 100 | 1.5 (1 to 2) |
| KFMΔSS7 | 100 | 1103 (1015 to 1191) | 100 | 6.6 (2 to 11) | 100 | 10.1 (0 to 22) | 86 | 1.0 (1 to 2) |
| KFMΔSS16 | 100 | 1074 (988 to 1134) | 100 | 6.4 (1 to 12) | 100 | 18.4 (1 to 36) | 80 | 1.0 (0 to 2) |
Incidence and frequency were determined 5 weeks after left ventricular injection of mice with C8161.9 cells that had been transfected with empty vector or with FLAG-tagged KISS1 containing (KFM) or lacking (KFMΔSS) the signal sequence and then infected with a green fluorescent protein–containing lentivirus. Three different C8161.9KFM clones (46, 57, 59) and three different C8161.9KFMΔSS clones (6, 7, 16) were used. At least 10 mice were used per experimental group.
Incidence reflects the percentage of mice with observable fluorescent metastases. Lung incidence represents the percentage of mice with metastatic foci _50 μm.
Frequency represents the number of metastatic foci per organ.
Statistically different (P<.001) compared with C8161.9vector for incidence (Fisher's exact test) and frequency (analysis of variance [ANOVA] followed by Holm–Sidak posttest).
Statistically significantly different (P<.01) compared with C8161.9vector for incidence (Fisher's exact test) and frequency (ANOVA followed by Holm–Sidak posttest).
Statistically significantly different (P<.05) compared with C8161.9vector for incidence (Fisher's exact test) and frequency (ANOVA followed by Holm–Sidak posttest).