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. Author manuscript; available in PMC: 2007 Mar 12.
Published in final edited form as: Mech Dev. 2006 Sep 10;124(1):11–22. doi: 10.1016/j.mod.2006.09.001

Figure 5.

Figure 5

Figure 5

Figure 5

Figure 5

Overexpression of GelA and MT-1 MMP leads to embryonic defects. 1 ng or 3 ng of mRNA encoding either GFP, GelA wild type, MT1-MMP wild type, or their mutants was injected into one blastomere of stage 2 embryos (day 0). Photographs were taken 3–4 days after injection (A and C) (with 3 ng mRNA). The percentage of embryos (relative to day 0) which were morphologically normal was determined each day and analyzed by ANOVA followed by Scheffe's posthoc test for embryos injected with 1 or 3 ng mRNA (B and D).

(A) and (B) Overexpression of both MT1-MMP wild type and inactive mutant E231A but not GFP caused deformation during early development. The quantification in (C) was based on 3 independent sets of experiments. Note that the wild type was more effective than the inactive mutant in affecting development.

(C) and (D) Overexpression of GelA wild type and autoactive mutants N100G and N100R caused severe deformation as compared to uninjected, GFP-injected, or E401A-injected animals. The quantification in (D) was based on 4 independent sets of experiments. Note that the autoactive mutants N100G and N100R were more effective than the wild type in affecting development.