Skip to main content
. 2007 Feb 28;104(10):3742–3746. doi: 10.1073/pnas.0607805104

Fig. 3.

Fig. 3.

Mechanistically distinct inhibitory effects by AptTBP-12 and AptTBP-101 on Pol II-dependent in vitro transcription. (A) Both AptTBP-12 and AptTBP-101 inhibit transcription equally effectively. The TATA-binding protein (TBP) concentration in the whole-cell extract is ≈20 nM. The concentration indicated is that of the aptamers. (B) The inhibitory effects of aptamers are reversed by excess TBP. Transcription in whole-cell yeast extracts by using pG5MLT (adenovirus major late promoter with 5X Gal4-binding sites upstream) in the presence of Gal4-VP16. Aptamer (50 nM) was added with template; TBP (500 nM) was added to extract before aptamer and template.