Skip to main content
. 2007 Feb 27;104(10):3747–3752. doi: 10.1073/pnas.0700087104

Fig. 3.

Fig. 3.

Binding of rGlyR3 to the celC promoter region as revealed by EMSA. All reactions contained 5 ng of a biotin-labeled 100-bp DNA fragment corresponding to the celC promoter region. Lane 1, no protein; lane 2, rGlyR3 (1 ng); lanes 3 and 4, cell lysate from the cellobiose-grown C. thermocellum culture (200 and 500 ng of protein, respectively); lane 5, cell lysate from the lichenan-grown C. thermocellum culture (120 ng of protein). The shifted band from lane 5 was excised and subjected to MALDI-TOF analysis, confirming the binding protein to be GlyR3.